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. 2026 Jan 16;13:1675742. doi: 10.3389/fpubh.2025.1675742

Table 3.

Descriptives of wastewater sampling and analysis methods of wastewater and environmental surveillance (WES) conducted in the included studies in the scoping review on public health actions in response to pathogen detection in wastewater.

Included studies N = 49 (%)
Wastewater sampling technique *
Composite sampling 33 (67%)
Grab sampling 14 (29%)
Passive sampling 3 (6%)
Vacuum sampling 1 (2%)
Wastewater sampling locations
Wastewater treatment plant 17 (35%)
Sewer manhole 17 (35%)
Sewer pipe 6 (12%)
Open drains, canals and ditches 4 (8%)
Lift station 2 (4%)
Septic tank 1 (2%)
Sewer ejector pumps 1 (2%)
Sewer pumping station 1 (2%)
Wastewater sampling frequency *
Daily 14 (29%)
Once or more times per week 28 (57%)
Less than once per week 8 (16%)
Not described 2 (4%)
Wastewater sampling period
1–100 days 12 (24%)
101–300 days 11 (22%)
≥301 days 20 (41%)
Not described 6 (12%)
Wastewater analysis types
Quantitative detection 35 (71%)
Qualitative detection 12 (24%)
Not described 3 (6%)
Wastewater analysis techniques *
Molecular 40 (82%)
Culture-based 6 (12%)
Sequencing-based 2 (4%)
Not described 3 (6%)
Wastewater analysis outcome measures
Viral load 27 (55%)
Negative, positive, inconclusive 17 (35%)
Virus type 2 (4%)
Fluorescence 1 (2%)
Not described 2 (4%)

*Multiple categories per included study.