Fig. 7.
Quantitative proteomic profiling of spinal cord tissue 7 days after SCI and treatment with PDA-HP hydrogel ± NIR irradiation. (A–D) Volcano plots of differentially expressed proteins (DEPs; |log2 FC| ≥ 1, FDR <0.05) for the indicated comparisons: (A) SCI vs. Sham, (B) PDA-HP/NIR vs. SCI, (C) PDA-HP vs. SCI, and (D) PDA-HP/NIR vs. PDA-HP. (E) Upset plot showing overlap of DEPs among the four comparisons. (F) Venn diagram of up-regulated proteins for each treatment relative to SCI. (G) Bar chart summarizing the number of up- and down-regulated proteins in each comparison. (H) Heat map of the top 50 most variable proteins (z-score) across all groups. Proteomic data were acquired by 4D-DIA LC-MS/MS (n = 4 biological replicates per group). Notably, SCI induced a marked up-regulation of HSP70, which was almost completely reversed by PDA-HP/NIR treatment.