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. 2025 Dec 12;302(2):111047. doi: 10.1016/j.jbc.2025.111047

Figure 2.

Figure 2

FasL increases apoptosis and cell death in the α2-6-sialoglycan deficient T cell model compared to other TNF receptor superfamily ligands.A, workflow for evaluating WT or ST6GAL1−/− Jurkat cell death induced by TRAIL, TNF-α, and FasL. Annexin V (recognizes exposed phosphatidylserine) and DAPI (intercalates DNA) report on apoptosis and cell death, respectively. B, flow cytometry plots for Annexin V and DAPI staining of WT or ST6GAL1−/− Jurkat cells treated with 50 ng/mL TRAIL, 100 ng/mL TNF-α, or 100 ng/mL FasL. Bottom left gates (blue) = live cells, bottom right gates (orange) = apoptotic cells, and top right gate (red) = dead cells. Percent values indicate the portion of total singlet cell events within each quadrant. C, D, and E, quantification of data from (B). F, immunoblot against cleaved (cl.) caspase 3 (18 kDa) with β-actin shown as loading control (45 kDa). For plots in C–E, results are reported as mean SD from three experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 ∗∗∗∗p < 0.0001, p > 0.05 = ns = not significant. Two-way ANOVA with Tukey post hoc test.