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. 2025 Dec 12;302(2):111047. doi: 10.1016/j.jbc.2025.111047

Figure 3.

Figure 3

α2-6-sialoglycan deficiency enhances localization dynamics of the Fas Receptor.A, workflow for evaluating surface presentation and spatial organization of FasR on WT or ST6GAL1−/− Jurkat cells via imaging flow cytometry. B, surface expression levels of FasR as a function of ST6GAL1 expression, exposure to FasL (100 ng/mL), and live vs. apoptotic status as measured by the BB515 channel on the imaging flow cytometer (same dataset as imaging data in subsequent panels). Gating scheme for live vs. apoptotic as in Figure 2B. FMO = fluorescent minus one staining control. C, Quantification of results from B. D, diffusivity of signal from the fluorescent anti-FasR antibody. E, representative individual cell images from the same groups of cells as reported in B. Data recorded via imaging flow cytometry. Grayscale layer corresponds to lightloss, which is analogous to a brightfield image of the cell. Green layer corresponds to the fluorescence signal from the anti-FasR antibody. F, Quantification of data from D. G, complementary fluoresence microscopy images for the experiment described in A. Scale bar, 10 μm. For plots in (C) and (F), results are reported as mean SD from three experiments. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, p > 0.05 = ns = not significant. Two-way ANOVA with Tukey post hoc test.