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. 2025 Dec 12;302(2):111047. doi: 10.1016/j.jbc.2025.111047

Figure 4.

Figure 4

Sialylation influences FasL mediated cell death by altering intracellular phosphorylation events.A, phosphoproteomics workflow for analysis of WT or ST6GAL1−/− Jurkat cells treated with FasL. B, Volcano plot of differentially phosphorylated peptides detected in ST6GAL1−/− relative to WT Jurkat cells treated with 100 ng/mL FasL. Phosphopeptide IDs that were compatible with RoKAI analysis are annotated in red. Companion plot for cells that were not treated with FasL is presented in Figure S4. C, differential activity of the kinases and phosphatases in ST6GAL1−/− relative to WT Jurkat cells identified via RoKAI analysis of the dataset from panel B. D, GO biological process terms associated with the kinases and phosphatases identified in C. Figure generated using ShinyGO V. 0.85 (55). All data is the result of four technical replicates submitted for phosphoproteomic analysis.