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. 2026 Jan 16;17:1737852. doi: 10.3389/fgene.2026.1737852

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Copyright © 2026 Ni, Gu, Wang, Zhang, Zhou, Zhang, Gao and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of miRNA-Seq and RT-qPCR expression for ssc-miR-127, ssc-miR-148a-3p, ssc-miR-146b, and ssc-miR-424-5p across months M1, M3, M6, and M8. Each panel shows bar graphs with miRNA expression and error bars, using orange for miRNA-Seq and blue for RT-qPCR. — Expressions of the DE miRNAs using miRNA-Seq and RT-qPCR. (A) DE miRNAs expression as detected by miRNA-Seq; (B) RT-qPCR analysis of selected DE miRNAs. Error bars indicate the mean ± standard deviation of three independent replicates.