Fig. 1. Phylogenetic position of the bee-associated species S. anthophorae sp. nov. and S. nidicola sp. nov. within the genus Streptomyces. (a) Genome tree created on DSMZ server, inferred with FastME 2.1.6.1 from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5, scale at top left. The numbers at nodes are GBDP pseudo-bootstrap support values >70% from 100 replications. New species are represented by coloured boxes and show distinction for the two new proposed species. Tree formatted in iTOL [67]. (b) Five-gene multilocus sequence alignment was performed using genes commonly used for phylogeny in existing Streptomyces literature (atpD, gyrB, recA, rpoB and trpB), alignment performed on MAFFT with MAFFT-L-INS-i. Conserved sites (2,278) were then used to construct a neighbour-joining tree using the Jukes–Cantor model. The numbers at nodes indicate bootstrap support values >60% from 1,000 replications. Green rectangle (top) outlines the clade of five isolates representing S. anthophorae sp. nov. with the two clusters labelled as clade 1 and clade 2. The red rectangle (centre) indicates the isolate representing S. nidicola sp. nov. Branch length indicates base changes per 100 bases, scale bar in bottom left.