Skip to main content
. Author manuscript; available in PMC: 2026 Jan 31.
Published in final edited form as: ACS Chem Biol. 2025 Dec 19;21(1):26–36. doi: 10.1021/acschembio.5c00639

Figure 3. Cell-free DNA cross-linking studies employing colibactin derivatives 6–12.

Figure 3.

Conditions: Linearized pUC19 DNA (14.3 nM, 38.5 μM in bp), 6–12 (1, 10, or 100 μM), 50 mM sodium citrate buffer (pH 5.0), 3 h, 37 °C. The DNA was denatured in the presence of 0.4% NaOH and analyzed by agarose gel electrophoresis (90 V, 1.5 h). See also Figure S2.