Skip to main content
. Author manuscript; available in PMC: 2026 Jan 31.
Published in final edited form as: ACS Chem Biol. 2025 Dec 19;21(1):26–36. doi: 10.1021/acschembio.5c00639

Figure 5. Cell cycle analysis.

Figure 5.

The percentage of cells in G1, S, and G2 cell cycle phases after treatment with different doses of colibactin 686 (9) is shown. Conditions: HeLa cells were incubated with colibactin 686 (9, 0.08–15,000 nM) for 4 h, washed, cultured an additional 20 h, fixed with paraformaldehyde and stained with HOECHST nuclear dye. Following immunofluorescent imaging, nuclei were segmented and integrated HOECHST intensity in individual nuclei was quantified using Metaxpress software. Histograms from DMSO-treated cells were used to identify the centers of the 2N and 4N DNA peaks and normalize the 2N DNA peak to 1 and the 4N DNA peak to 2. Cells were then classified by normalized log2 DNA content as G1 (0.75–1.25), S (1.25–1.75), or G2 (1.75–2.5) phase cells. The percentage of cells within each phase of the cell cycle was determined for each treatment condition. Columns indicate the mean; error bars indicate SD; n = 6 independent wells analyzed.