Table 2.

Histopathological findings across control and treatment groups (n = 10)

Parameters	Control	MTX	MTX + β-Sitosterol
Hepatocyte necrosis	0.1 ± 0.1	1.3 ± 0.1**	0.6 ± 0.2#
Fibrogenic alterations	0.2 ± 0.1	2.1 ± 0.3**	1.3 ± 0.3#
Cellular infiltration	0.2 ± 0.1	1.6 ± 0.2**	0.9 ± 0.2#

Data are shown as mean ± SEM. Control group: only vehicle (1 mL/kg/day); MTX group: MTX (20 mg/kg, single dose, i.p.) and vehicle (1 mL/kg/day); MTX + β-sitosterol group: MTX (20 mg/kg, single dose, i.p.) and β-sitosterol (80 mg/kg/day)

Statistical analyses were performed using Kruskal-Wallis test

^**p < 0.001 distinct from control group

^#p < 0.05 distinct from MTX group