Table 2.

RNA quality analysis after poly(A) depletion.

Sample		c (ng/μL)	% of total RNA	RQI	A260/A280a	A260/A230a
1	Total RNAb	462.0	100	10	1.99	2.39
	Poly(A)-depletedc	157.5	76.71	10	2.04	2.19
	Eukaryotic mRNAd	15.5	0.84	-	2.07	0.98
2	Total RNA	468.0	100	10	2.07	2.43
	Poly(A)-depleted	129.0	55.13	10	2.04	2.31
	Eukaryotic mRNA	22.9	0.98	-	2.07	1.36
3	Total RNA	1173.2	100	10	2.07	2.35
	Poly(A)-depleted	190.7	36.56	10	2.04	2.17
	Eukaryotic mRNA	21.9	0.47	-	2.13	1.47

^aThe A260/A280 and A260/A230 ratios were determined with a NanoVue spectrophotometer.

^bTotal RNA was extracted from 0.5 × 10⁷ cells/mL cultured for 6 days (samples 1, 2) or 9 days (sample 3) in 12-well plates with the miRNeasy Mini Kit. Poly(A) depletion was performed and the resulting poly(A)-depleted RNA and eukaryotic mRNA were recovered. Total RNA was analyzed with the Experion StdSens Kit using the eukaryotic total RNA protocol on the Experion Automated Electrophoresis Station. The total RNA of samples 1 and 3 was diluted 1:1 before measuring.

^cPoly(A)-depleted RNA (1:100 diluted) was analyzed with the HighSens Kit using the eukaryotic total RNA protocol on the Experion Automated Electrophoresis Station.

^dEukaryotic mRNA was analyzed with the Experion StdSens Kit using the eukaryotic total RNA protocol on the Experion Automated Electrophoresis Station. A hyphen (−) indicates that RQI was not determined due to selection of the mRNA protocol.