Table 2. Recombinant expression and purification details for TaXPD.

Source organism	T. acidophilum
DNA source	Gene synthesis and cloning
Cloning vector	pET-28a
Expression host	E. coli BL21(DE3) Rosetta
Complete amino-acid sequence of the construct produced†	HHHHHHSSGLEVLFQGPHRGSEFELMYENRQYQVEAIDFLRSSLQKSYGVALESPTGSGKTIMALKSALQYSSERKLKVLYLVRTNSQEEQVIKELRSLSSTMKIRAIPMQGRVNMCILYRMVDDLHEINAESLAKFCNMKKREVMAGNEAACPYFNFKIRSDETKRFLFDELPTAEEFYDYGERNNVCPYESMKAALPDADIVIAPYAYFLNRSVAEKFLSHWGVSRNQIVIILDEAHNLPDIGRSIGSFRISVESLNRADREAQAYGDPELSQKIHVSDLIEMIRSALQSMVSERCGKGDVRIRFQEFMEYMRIMNKRSEREIRSLLNYLYLFGEYVENEKEKVGKVPFSYCSSVASRIIAFSDQDEEKYAAILSPEDGGYMQAACLDPSGILEVLKESKTIHMSGTLDPFDFYSDITGFEIPFKKIGEIFPPENRYIAYYDGVSSKYDTLDEKELDRMATVIEDIILKVKKNTIVYFPSYSLMDRVENRVSFEHMKEYRGIDQKELYSMLKKFRRDHGTIFAVSGGRLSEGINFPGNELEMIILAGLPFPRPDAINRSLFDYYERKYGKGWEYSVVYPTAIKIRQEIGRLIRSAEDTGACVILDKRAGQFRKFIPDMKKTSDPASDIYNFFISAQAREKYGA

^†The bold amino-acid sequence highlights the 19 amino acids present only in the long TaXPD construct. Underlined residues indicate the extra residues from the vector for N-terminal His-tag attachment.