TABLE 1.
ASH1 mRNA localization in cells expressing She2p mutants
| Plasmid/She2pa | ASH1 mRNA localizationb |
| pRL199/She2-wt | 48% |
| pRL324/She2p-N36S | 0% |
| pRL745/She2p-R43K | 54% |
| pRL740/She2p-R43A | 18% |
| pRL746/She2p-R44K | 52% |
| pRL741/She2p-R44A | 2% |
| pRL747/She2p-R49K | 46% |
| pRL742/She2p-R49A | 24% |
| pRL748/She2p-R52K | 0% |
| pRL743/She2p-R52A | 2% |
| pRL322/She2p-R63K | 0% |
| pRL744/She2p-R63A | 0% |
aThe indicated plasmids were transformed into strain YLM777 along with plasmid C3431 (YEplac195/ASH1).
bFifty anaphase cells with signal for ASH1 mRNA were counted. The percent of cells with localized ASH1 mRNA is indicated.