TABLE 2.
Substitutions in blaTEM genes and derived β-lactamases
| Region and nucleotide no.a (amino acid no.)b | Nucleotide (amino acid) in the following gene (protein):
|
|||||||
|---|---|---|---|---|---|---|---|---|
| blaTEM-1A | blaTEM-1B | blaTEM-2 | blaTEM-45 | blaTEM-68 | blaTEM-82 | blaTEM-83 | blaTEM-103 | |
| Promoter region | P3 | P3 | Pa and Pb | P4 | Pa and Pb | P4 | P3 | |
| 32 | C | C | T | C | NDd | T | C | C |
| 162 | G | G | G | T | ND | G | T | G |
| 175 | A | G | A | A | ND | A | A | G |
| Coding region | ||||||||
| 226c | C (Phe) | T | C | C | T | C | C | T |
| 317 (39) | C (Gln) | C | A (Lys) | C | C | C | C | C |
| 346c | A (Glu) | A | G | G | A | A | G | A |
| 407 (69) | A (Met) | A | A | A | A | G (Val) | T (Ile) | A |
| 409 (69) | G (Met) | G | G | T (Leu) | G | G | G | G |
| 436c | C (Gly) | T | T | T | T | C | T | T |
| 604c | G (Ala) | T | G | G | G | G | G | T |
| 682c | T (Thr) | T | C | C | C | T | C | T |
| 697 (165) | G (Trp) | G | G | ND | G | G | T (Cys) | G |
| 914 (238) | G (Gly) | G | G | ND | A (Ser) | G | G | G |
| 917 (240) | G (Glu) | G | G | ND | A (Lys) | G | G | G |
| 925c | G (Gly) | G | A | A | A | G | A | G |
| 929 (244) | C (Arg) | C | C | ND | C | C | C | C |
| 990 (265) | C (Thr) | C | C | ND | T (Met) | C | C | C |
| 1020 (275) | G (Arg) | G | G | A (Gln) | T (Leu) | A (Gln) | A (Gln) | T (Leu) |
| 1022 (276) | A (Asn) | A | A | ND | A | A | A | A |
| Source or reference | 18 | 8 | 8 | 4 | 7 | 11 | 11 | This study |