TABLE 3.

K_i and K_i/K_m of wild-type and mutant HIV-1 RT enzymes

HIV-1 RT	Tenofovir-PP		ddATP		Carbovir-TP		DXG-TP
	Ki (μM)a (fold)b	Ki/Km (fold)c	Ki (fold)	Ki/Km (fold)	Ki (μM) (fold)	Ki/Km (fold)	Ki (μM) (fold)	Ki/Km (fold)
Wild type	0.155 (1.0)	0.46 (1.0)	0.024 (1.0)	0.072 (1.0)	0.181 (1.0)	1.05 (1.0)	0.008 (1.0)	0.05 (1.0)
K65R	1.040d (6.7)	2.67 (5.8)	0.173e (7.2)	0.441 (6.1)	0.357 (2.0)	1.42 (1.3)	0.033e (4.1)	0.13 (2.8)
K65R+M184V	0.824d (5.3)	1.62 (3.5)	0.383d (16)	0.690 (9.6)	0.807d (4.5)	2.45 (2.3)	0.033e (4.1)	0.10 (2.2)

^aK_i values are averages for three to six experiments, with average standard errors of 0.067 for tenofovir-PP, 0.013 for ddATP, 0.059 for carbovir-TP, and 0.003 for DXG-TP.

^bFold change in K_i value from that for the wild type.

^cFold change in K_i/K_m value from that for the wild type.

^dP < 0.001 compared to the K_i for the wild type by two-tailed Student's t test.

^eP < 0.05 compared to the K_i for the wild type by two-tailed Student's t test.