TABLE 4.
Processivity of HIV-1 wild type and mutant RT enzymes
| HIV-1 RT | Processivity with poly(rA)-oligo(dT)15 template
|
Processivity with HIV RNA template (%)c | |||
|---|---|---|---|---|---|
| Median length extendeda
|
Peak length extendedb
|
||||
| 0.5 μM dTTP | 0.05 μM dTTP | 0.5 μM dTTP | 0.05 μM dTTP | ||
| Wild type | 50 | 30 | 40 | 20 | 100 |
| K65R | 52 | 24 | 40 | 10 | 69 |
| K65R+M184V | 38 | 21 | 35 | 10 | 31 |
a
Median length extended (in nucleotides) measured as the median length of extension products determined from regression analysis of band intensity measured by using 10-nt intervals.
b
Peak of the percentage of products generated versus length of product (in nucleotides) measured by using 10-nt intervals.
c
Percentage of full-length cDNA in the presence of a quenching reagent/total activity without a quenching reagent normalized to the value for the wild type (WT), which was set at 100%.