Figure 7.

5-azaC inhibition of DNMT1 dynamics occurs in the S phase of the cell cycle. (A) Schematic of double thymidine block protocol used to synchronize cells, followed by either DMSO or 4 μM 5-azaC treatment for 4 h. (B–I) Spot-on analysis of cells synchronized to S phase and treated with 5-azaC. (B–C) Pie charts showing the distribution of DNMT1 molecules following DMSO and 5-azaC treatment in either asynchronous or synchronized (4 h post-THY) cells. (D–I) Violin plots showing the diffusion coefficients and distribution of DNMT1-Halo in 40–65 cells across three replicates. Student t-test, two-tailed, **** P < 0.0001, ns = not significant.