FIG. 3.

The T. thermophilus ribosome with tRNAs (PDB accession no. 1GIX and 1GIY [22]) emphasizing the putative binding site of avilamycin. (A) The rRNA and protein backbone are shown in grey, and tRNA in the A-, P-, and E-site is shown in red, purple and orange. Helix 89, where mutations confer avilamycin resistance, is indicated in green, and helix 91 is indicated in blue. (B) Helix 89 (green) and helix 90 (blue) region with the nearby L16 (yellow) and tRNA in the A-site (red). The positions of the mutations selected by avilamycin are indicated by red balls, and the avilamycin footprints are indicated by black balls. The grey background tubing is the backbone of domain V of 23S rRNA. The molecular visualization was done using VMD (10).