TABLE 1.
Cumulative incidence of bacteria isolated from tissues of B6D2F1/J mice after irradiation, i.t. challenge on day 4 with B. anthracis Sterne spores, and during treatment with an antimicrobial agent (experiment 2)a
| Bacterium isolated | No. of mice with bacteria in tissues/ no. of mice examined (%)
|
|||
|---|---|---|---|---|
| Control | PEN Gb s.c. | OFX p.o. | Total | |
| B. anthracis only | 6/16 (38) | 5/23 (22) | 3/21 (14) | 14/60 (23) |
| Other species only | 0/16 (0) | 1/23 (4) | 1/21 (5) | 2/60 (3) |
| Gram positive | 0 | 0/1 | 1/1 | |
| Gram negative | 0 | 1/1 | 0/1 | |
| Mixed (B. anthracis + other) | 5/16 (31) | 10/23 (43) | 8/21 (38) | 23/60 (38) |
| Other, gram positive | 5/5 | 7/10 | 8/8 | |
| Other, gram negative | 4/5 | 9/10 | 0/8 | |
| None | 5/16 (31) | 7/23 (30) | 9/21 (43) | 21/60 (35) |
a
The mice were irradiated with 7 Gy of 60Co γ radiation and challenged with 3.3 × 108 CFU of B. anthracis Sterne spores. Lung, spleen, or heart blood was aseptically removed from mice euthanized on schedule on days 5, 7, 9, 12, and 14. Therapy was begun 7.5 h after challenge. The numerators show the proportion of animals from which general categories of bacteria were isolated relative to therapeutic agent or control. The numerators were differentiated further between gram-positive and gram-negative bacteria.
b
Dose, 125 mg of PEN G/kg.