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Effects of dopamine on crosslinking of selected D2R TM4 cysteine mutants with copper phenanthroline. (A) I1584.48C and W1604.50C were crosslinked by the concentrations of CuP indicated in Fig. 3B in the absence or presence of 10 μM dopamine and detected by immunoblotting. (B) The sulpiride- and dopamine-induced changes in crosslinking fraction for each mutant are shown as mean ± SEM (n = 3-5) for dopamine (open bars) or sulpiride (filled bars). DA, dopamine.
