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. 2005 Nov 29;3(12):e415. doi: 10.1371/journal.pbio.0030415

Figure 8. Compensatory Refeeding and Neuropeptide mRNA levels in Pomc-Specific and Agrp-Specific Tfam Deficiency.

Figure 8

(A–D) 24-h compensatory refeeding after a 48-h fast was measured as described in Materials and Methods; data are shown either as (A and B) the ratio of food consumed over 24 h (refeeding) to normal daily food intake (averaged over 7 h prior to food deprivation), or as (C and D) percentage of weight recovery after 24 h of refeeding. For the Pomc-Tfam experiment (A and C), number of animals used was n = 23 (control) and n = 13 (Pomc-Tfam mutant); for the Agrp-Tfam experiment (B and D), number of animals used was n = 21 (control) and n = 15 (Agrp-Tfam).

(E) The same refeeding defects were observed in Pomc-Tfam mutants after corticosterone replacement (control, n = 9; Pomc-Tfam mutant, n = 6).

(F) Expression of Pomc, Agrp, and Npy in Pomc-Tfam mutants. Mice were fasted for 48 h 10 d after implanting corticosterone pellets, and expression of Pomc, Agrp, and Npy in the hypothalamus was measured by semi-quantitative RT-PCR. Values are shown as relative levels compared to free-fed controls. Numbers of animals used were n = 5 (control fed); n = 5 (control fasted); n = 3 (mutant fed); and n = 3 (mutant fasted).

*, p ≤ 0.05; **, p ≤ 0.01. Error bars = standard error of the mean.