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. 2005 Nov 29;3(12):e416. doi: 10.1371/journal.pbio.0030416

Figure 2. Assessment of Chromosomal Stability in Interphase Nuclei of Parental HCT116 Cells and Chromosomally Stable hSecurin−/− Cells Using Centromere-Specific Probes for Chromosomes 7, 8, 11, and 17.

Figure 2

(A and B) Representative interphase FISH images of parental HCT116 (hSecurin+/+) (A) and hSecurin−/− (B) cell nuclei after hybridization of a four-color probe set consisting of centromere probes for chromosomes 7 (Cy5.5; purple), 8 (FITC; green), 11 (Cy5; blue), and 17 (Cy3; yellow). In each nucleus, two signals are visible for each probe.

(C and D) Graphic summary of chromosome gains and losses in parental HCT116 (C) and hSecurin−/− (D) cells. The percentage of signals per nucleus for chromosomes 7, 8, 11, and 17 was determined from 300 cells of each genotype (100 cells each in three separate experiments).