Figure 2. The Ankyrin-B-Based Complex of NKA, NCX1, and InsP₃R Is Localized in a Specialized T-Tubule Microdomain of Cardiomyocytes.

Adult cardiomyocytes were labeled with indicated antibodies. 3D reconstructions of each cell (A, differential interference contrast) are shown for each combination. Staining pairs include (B) DHPR (red) and RyR (green), (C) DHPR (red) and NCX (green), (D) NCX (red) and NKA alpha1 (green), (E) NCX (red) and NKA alpha 2 (green), (F) NKA alpha 1 (red) and NKA alpha 2 (green), (G) InsP₃R (red) and RyR (green), and (H) InsP₃R (red) and NCX (green). Voxel co-localization was performed for non-sarcolemmal voxels. T-tubule/Z-lines are indicated by white arrows. Scale bar = 5 μm.