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letter
. 2002 Nov;46(11):3690–3691. doi: 10.1128/AAC.46.11.3690-3691.2002

FIG. 1.

FIG. 1.

(A) Comparison of the mega of S. salivarius (GenBank accession no. AJ3188993) and S. pneumoniae (GenBank accession no. AF274302) in a dot plot analysis. IR, inverted repeats described by Gay and Stephens (3). (B) Identification of the S. pneumoniae R800 chromosomal DNA fragment containing the S. salivarius mega by digestion with SmaI, pulsed-field gel electrophoresis, and Southern hybridization. Lanes M, Lambda Ladder PFG Marker (in kilobases) (Biolabs); lanes 1, S. pneumoniae R800; and lanes 2, R800-transformant RSp6-II. Arrows indicate the fragment into which mega and adjacent sequences integrated.