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. 2000 Aug 1;67(3):610–622. doi: 10.1086/303050

Figure 1.

Figure 1

Diagram representing hybridization selection enrichment for amplicons containing telomere-junction fragments with homogenous arrays of TTAGGG repeats only. Size fractionation for MboI-digested genomic DNA >5 kb gives a 50–100-fold enrichment for long MboI fragments, including telomeres. After amplification of these products by telomere-anchored PCR with linker primer SauL-A and telomere primer TelC, a two-step hybridization selection is done. In step 1, telomere repeat arrays containing variant-repeat types TGAGGG, TCAGGG, and TTGGGG are partially removed by hybridization to the variant-repeat filter. In step 2, the TTAGGG filter is used to select amplicons containing (TTAGGG)n repeats, and the selected amplicons are reamplified by telomere-anchored PCR, directionally cloned, and picked into an ordered array library. The combined effect of each step is to enrich for homogenous arrays of TTAGGG repeats. Blackened arrowheads denote TTAGGG repeats; unblackened arrowheads, variant repeats TGAGGG, TCAGGG, and TTGGGG.