Table 1.
Primers Used for PCR Amplification of Junction Fragments
| der(11) Primers |
der(22) Primers |
|||
| PCR | Name | Sequence | Name | Sequence |
| Firsta | c14irev | 5′-GGAAGTTAGAGAAAACTGAGAA-3′ | c14hfor | 5′-AACACTCCCACTGACAGCTA-3′ |
| JF22.2 | 5′-CCTCCAACGGATCCATACT-3′b | JF22.2 | 5′-CCTCCAACGGATCCATACT-3′b | |
| Secondc | JFN11 | 5′-CAGAAAGGGAGAGCATGTAG-3′ | JFN11.2 | 5′-GGTTGAAGAATCTTGGCTGG-3′ |
| JFN22 | 5′-CGTTGAAGGATGCAGGATGT-3′ | JFN22 | 5′-CGTTGAAGGATGCAGGATGT-3′ | |
a
Conditions are as follows: 5 cycles of 94°C for 30 s, 58°C for 30 s, and 72°C for 1 min, followed by 35 cycles of 94°C for 30 s, 56°C for 30 s, and 72°C for 1 min.
b
Corresponds to primer “c” in the study by Kehrer-Sawatzki et al. (1997).
c
Conditions are as follows: 35 cycles of 94°C for 15 s, 58°C for 15 s, and 72°C for 30 s.