TABLE 3.
Days p.i. | No. of birds with infection at:
|
Virus isolation | |||
---|---|---|---|---|---|
1 day old
|
2 wk old
|
||||
Sham inoculated | Infected | Sham inoculated | Infected | ||
2 to 5 | 0/4 (0) | 4/4 (3) | 0/2 (0) | 2/4 (1) | Yes |
6 to 8 | 0/4 (0) | 4/4 (4) | 0/2 (0) | 2/4 (2) | Yes |
10 to 14 | 0/4 (0) | 2/4 (0) | 0/2 (0) | 1/4 (0) | No |
Total | 0/12 (0) | 10/12 (7) | 0/6 (0) | 5/12 (3) |
Data are presented as number of animals positive for aMPV RNA by RT-PCR/total number tested. The number of RNA-positive birds also positive for viral antigens by immunohistochemistry is presented in parentheses. Whether or not the virus isolation was successful, which was only attempted in birds inoculated at 2 weeks old, is also indicated.