FIG. 4.

Binding of scFvs to rWNV-E. (a) Binding of scFvs to rWNV-E as measured by ELISA. ELISA plates were coated with rWNV-E (100 ng/well) overnight and incubated with serial dilutions of antibodies. Anti-His-HRP (1:4,000) was used as a secondary antibody, and the plates were developed and read at 450 nm (OD₄₅₀). (b) rWNV-E (10 μg/gel) was run on a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel under reducing (in the presence of 2-mercaptoethanol) or nonreducing conditions and blotted to nitrocellulose. Blots were incubated with scFvs (1 μg/ml), and binding was detected by incubation with anti-His-HRP (1:4,000). Lane Ab refers to control for background anti-His-HRP antibody binding to rWNV-E.