FIG. 8.

H1IT Tax activity is not repressed by p30. 293T cells were transfected with either wild-type HTLV-1 or H1IT proviral DNA along with the Tax reporter LTR-1-luciferase in the presence or absence of a p30 expression plasmid. CMV-βgal was used to control for transfection efficiency. Cell lysates were assayed 48 h posttransfection for Tax activity. The data shown are representative of three independent experiments. As previously reported, p30 is able to inhibit Tax activity in HTLV-1, whereas H1IT had an opposite effect, with Tax activity showing a two- to threefold induction in the presence of p30.