FIG. 3.

Amplification of BaMV40A and its derivatives in N. benthamiana protoplasts. Representative experiments that contributed to the quantitative data in Table 1 are shown. (A) Northern blot analysis of the plus-strand genomic (G; 6.4 kb) and two subgenomic (SG; 2.0 and 1.0 kb) RNAs. RNAs were probed with a ³²P-labeled RNA transcript complementary to a 0.6-kb region at the 3′ end of genomic RNA. (B) Northern blot analysis of the minus-sense antigenomic (G; 6.4 kb) RNAs. RNAs were probed with a ³²P-labeled RNA transcript complementary to a 0.7-kb area at the 3′ end of minus-sense RNA. Lane dIL represents the mutant with a deletion at the 3′ side of the internal loop, and lane c represents 1 ng of virion RNA loaded as a negative control. The other mutants are indicated at the top of each lane. WT, wild type.