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In vitro translation assay of pBaMV40A and its derivatives. In vitro translation was performed in rabbit reticulocyte lysate (A) or wheat germ extract (B) with plasmid template DNAs of pBaMV40A and its derivatives. The products were separated on 8% SDS-PAGE and analyzed by a phosphorimager (Fujifilm BAS 1500). The molecular masses of the markers are indicated on the left. The arrow indicates the position of the replicase. WT, wild type.
