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Schematic representation of the Δ4SHIVKU2 plasmid used in this study. This plasmid has tat, rev, env, and vpu genes from HIV-1 strain HXB2 and gag, pro, and nef from HIV-1 strain SF2. The 5′ LTR, vpr, and vpx are from SIVmac 239. Sequences from the rt, int, and vif genes and the 3′ LTR were deleted to make this plasmid noninfectious, and poly(A) sequences from SV40 were added.
