FIG. 10.

Cell phenotypes responsible for producing IFN-γ in mice injected with vaccine or with vaccine and GM-CSF DNA. Numbers of IFN-γ-producing CD4⁺ or CD8⁺ T cells per million splenocytes, at 8 and 12 weeks, from groups of mice that were coinjected with either vaccine DNA or vaccine and GM-CSF DNAs during the boost are shown. The CD4⁺ T-cell-depleted fraction had significantly higher numbers of IFN-γ-producing cells than the CD8⁺ T cell fraction. The group that received the GM-CSF DNA boost had significantly higher (P < 0.05) ELISPOT counts in both cell subsets than the group that received only vaccine DNA. Error bars indicate standard deviations.