FIG. 2.
Immunoprecipitation of major viral proteins in HEK 293 cells transfected with Δ4SHIVKU2 DNA. Viral proteins precipitated by an HIV patient serum and by anti-Vpu and anti-Nef antibodies from transfected HEK 293 cells at 36 h posttransfection are shown. Both Env and Gag proteins with their precursors and Nef and Vpu were precipitated from the cell lysate fraction. Env gp120 and gp41 and Gag p55 and p24 were precipitated from the supernatant fluid (Sup). Con, cell lysate from nontransfected HEK 293 control cells.