FIG. 4.
Histological examination of muscles coinjected with pGM-CSF and Δ4SHIVKU2 DNA. The coinjection of pGM-CSF DNA elicited massive mononuclear infiltration at the injected sites that resulted in a larger infiltrate than was elicited by vaccine DNA alone (Fig. 3). Panel A shows no infiltration in muscle sections from control mouse. Panel B shows a representative area of mononuclear infiltration in the muscle coinjected with pGM-CSF DNA at day 7 postinjection. Panel C shows that the majority of the cells in the infiltrates in the muscle coinjected with pGM-CSF and vaccine DNA were macrophages.