FIG. 5.
Immunohistochemical localization of Gag p24 in muscles of mice injected with Δ4SHIVKU2 DNA. Gag p24 was localized in the sarcoplasmic reticulum of skeletal muscle cells and in the cytoplasm of infiltrating mononuclear cells in the muscle. Panel A shows Gag p24 in the cytoplasm of some of the infiltrating cells at day 3. Panel B shows Gag p24 in the sarcoplasmic reticulum of skeletal muscle cells (arrowheads). Panels C and D show Gag p24 in the sarcoplasmic reticulum of skeletal muscle cells and in the cytoplasm of infiltrating mononuclear cells (arrowhead) at day 5. Panels E and F show Gag p24 in the sarcoplasmic reticular sacs (closed arrowheads) and in the cytoplasm of mononuclear cells (open arrowhead) at day 7. Panels G and H show p24 in muscles at days 10 and 12 postinjection. Panel I shows Gag p24 expression peaking in the skeletal muscle cells at day 7 after the second injection of vaccine DNA.