Skip to main content
. 2005 Dec;79(23):14756–14768. doi: 10.1128/JVI.79.23.14756-14768.2005

FIG. 6.

FIG. 6.

The amino acids of the C-terminal region important for IFN-induced signaling and STAT degradation. (A) V-specific regions of hPIV2 and hPIV4A V proteins. The symbols below the sequence indicate the positions of conserved cysteine (asterisks) residues, tryptophan (or tyrosine) (squares) residues, and insertion of point mutations. (B) Schematic diagram of the chimera V proteins described in the legend to Fig. 4A. The chimera proteins are named for amino acid numbers of the N terminus of hPIV2/hPIV4A (PIV2-aa/4) or the amino acid number of the substitution [PIV2+4(substitution number)] or point mutation (+mutation). The asterisk indicates the position of the mutated residue. Inhibition of IFN signaling and degradation of STAT are summarized on the right panel. (C) Effects of V proteins on IFN-α-stimulated gene activation. A reporter gene assay with luciferase was performed as described in the legend to Fig. 4B. Chimera 1, PIV2; chimera 2, PIV4; chimera 3, PIV2-177/4; chimera 4, PIV2-195/4; chimera 5, PIV2-209/4; chimera 6, PIV2-209/4+K220S; chimera 7, PIV2+4(178-195); chimera 8, PIV2+4(196-208); and chimera 9, PIV2+4(196-208)+E211F. (D) STAT1 and STAT2 levels in HeLa cells constitutively expressing V proteins. Western blotting was performed as described in the legend to Fig. 4C. The asterisk on the right indicates a cross-reacting host band. Numbers under the figure correspond to chimera numbers.