FIG. 1.

MIP-3α expression in Poly(I) · Poly(C)-treated control and E6/E7-expressing keratinocytes measured by real-time qPCR and ELISA. Primary human foreskin keratinocytes stably expressing vector alone (Babe) or HPV-16 E6/E7 (E6/E7) were treated with 100 μg/ml Poly(I) · Poly(C) for 16 h. Following treatment, cells were washed and refed with minimal EpiLife for 4 h. (A) RNA was harvested from the cells, and mRNA levels for MIP-3α were determined by real-time qPCR. Results are normalized to GAPDH and expressed as the means ± standard deviations (SD) of three experiments using three independent cell lines (* P < 0.001). (B) Culture supernatants were harvested, and levels of secreted MIP-3α were measured by ELISA. Results are expressed as the means ± SD of three experiments using three independent cell lines (* P < 0.001).