FIG. 4.

Effect of E6 and E7 on MIP-3α mRNA and protein levels. Primary human foreskin keratinocytes stably expressing vector alone (Babe), HPV-16 E6/E7 (E6/E7), HPV-16 E6/E7stop (E6), or HPV-16 E6stop/E7 (E7) were treated with 100 μg/ml Poly(I) · Poly(C) for 16 h. Following treatment, cells were washed and refed with minimal EpiLife for 4 h. (A) RNA was harvested from the cells, and mRNA levels for MIP-3α were determined by real-time qPCR. Results are expressed as the means ± SD of three experiments using three independent cell lines (* P < 0.001). (B) Culture supernatants were harvested, and levels of secreted MIP-3α were measured by ELISA. Results are expressed as the means ± SD of three experiments using three independent cell lines (* P < 0.001).