FIG. 4.

Role of capsid RGD in neutrophil-adenovirus interaction. (A) Transduction efficiency of Cy2-labeled and unlabeled AdL.PB* compared to AdLuc in REC cells. (B) Neutrophils were incubated with Cy2-AdLuc (open bars) or Cy2-AdL.PB* (closed bars) and fluorescence analyzed at 20 to 60 min. For AdLuc versus AdL.PB* at 20 min, P < 0.05 (*); n = 3. (C) Neutrophil L-selectin expression in response to Ad vectors. Neutrophils were incubated with vehicle (open bars), unlabeled AdLuc (hatched bars), or AdL.PB* (closed bars). L-selectin expression was determined by flow cytometry at 20 to 60 min For AdLuc or AdL.PB* versus vehicle, P < 0.05 (*); n = 5. Error bars indicate standard deviations.