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. 2005 Dec;79(23):14622–14631. doi: 10.1128/JVI.79.23.14622-14631.2005

FIG. 5.

FIG. 5.

Role of serum factors in neutrophil-adenovirus interaction. (A) Cy2-AdLuc was incubated with neutrophils and modified human serum. Cells were analyzed by flow cytometry at 20 (open bars), 40 (hatched bars), and 60 (closed bars) min. −Serum, no serum; CHS, complete human serum; −IgG, IgG-depleted human serum; HI HS, heat-inactivated human serum; −IgG HI HS, IgG-depleted heat-inactivated human serum; HI BS, heat-inactivated bovine serum. For CHS versus relevant time points from subsequent treatment groups, P < 0.01 (**); n = 3 or 4. (B) Cy2-AdLuc was incubated with neutrophils in the presence of EGTA (blocks classical complement pathway) or 50°C heat-inactivated human serum (blocks alternative complement pathway). Cells were analyzed by flow cytometry at 20 (open bars), 40 (hatched bars), and 60 (closed bars) min. For CHS versus corresponding EGTA treatment time points, P < 0.05 (*) or P < 0.01 (**); n = 3 or 4. Error bars indicate standard deviations.