FIG. 6.
Role of complement and Fc receptors in neutrophil-adenovirus interaction. (A) Cy2-AdLuc was incubated with neutrophils in the presence of blocking CD35, CD11b, CD18, or isotype control antibodies. Cells were analyzed by flow cytometry at 20 (open bars), 40 (hatched bars), and 60 (closed bars) min. For anti-CD35 versus isotype control, P < 0.05 (*); n = 3 to 5. (B) Cy2-AdLuc was incubated with neutrophils in the presence of blocking CD32, CD16, mouse IgG1, or mouse IgG2a antibodies. Cells were analyzed by flow cytometry at 40 min. For treatment groups versus IgG1 control, P < 0.05 (*) and P < 0.01 (**); n = 6. For combined Fc receptor blockade versus individual blockade, P = not significant (NS). (C) Effect of combined complement and Fc receptor blockade. Cy2-AdLuc was incubated with neutrophils in the presence of blocking CD35, CD32, CD16, mouse IgG2a, or control mouse IgG1 antibodies. Cells were analyzed by flow cytometry at 40 min. For CD35 blockade or combined receptor blockade versus IgG1 control, P < 0.05 (*) and P < 0.005 (***), respectively; n = 6. For combined CD35 and Fc receptor blockade versus CD35 alone, P < 0.05 (*); n = 6. Error bars indicate standard deviations.