TABLE 2.
Total and differential counts in BAL specimens following infection with 1918 recombinant viruses
| Infectiona | Day p.i. | Total cell yield (× 105)b | Mean cell no. (± SE)/mousec
|
||
|---|---|---|---|---|---|
| Neutrophils (× 103) | Lymphocytes (× 104) | Macrophages (× 104) | |||
| rTx/91 | 0 | 1.7 | 10.2 ± 4.9 | 5.6 ± 0.9 | 10.5 ± 0.9 |
| 1918 HA/NA:Tx/91 | 0 | 1.8 | 10.8 ± 4.3 | 5.9 ± 1.2 | 10.9 ± 1.2 |
| rTx/91 | 0.75 | 2.3 | 12.8 ± 3.1 | 6.7 ± 0.6 | 14.9 ± 1.5 |
| 1918 HA/NA:Tx/91 | 0.75 | 2.9 | 31.9 ± 6.6 | 10.4 ± 0.9 | 15.4 ± 1.4 |
| rTx/91 | 3 | 2.7 | 27 ± 5.1 | 7.5 ± 2.1 | 16.7 ± 1.7 |
| 1918 HA/NA:Tx/91 | 3 | 5.0 | 60 ± 19 | 15.0 ± 2.3 | 28.5 ± 3.6 |
| rTx/91 | 5 | 3.7 | 51.8 ± 10.5 | 15.4 ± 1.5 | 15.9 ± 1.9 |
| 1918 HA/NA:Tx/91 | 5 | 7.8 | 249.6 ± 24.2 | 15.6 ± 1.8 | 37.4 ± 3.7 |
| rTx/91 | 7 | 5.8 | 69.6 ± 26.6 | 22.0 ± 1.5 | 29.0 ± 2.2 |
| 1918 HA/NA:Tx/91 | 7 | 12.1 | 798.6 ± 73.2 | 13.3 ± 4.2 | 27.8 ± 5.5 |
| 1918 HA/NA:Tx/91, RB6-8C5 treatedd | 7 | 6.7 | 1.2 ± 2.6 | 27.8 ± 1.6 | 47.3 ± 1.7 |
| 1918 HA/NA:Tx/91, L-Cl2MBPe | 7 | 9.3 | 856 ± 65.6 | 29.2 ± 1.4 | 1.8 ± 1.6 |
a
Mice were i.n. infected with 106 PFU of the indicated viruses.
b
BAL specimens were collected from five mice per group on the days indicated. Data are the mean absolute cell numbers in the BAL specimens.
c
In each differential from five mice per group. Differential counts were performed on LeukoStat (Fisher Scientific)-stained cytospins.
d
For neutrophil depletion, mice received repeated i.p. injections of MAb RB6-8C5 beginning 5 h before i.n. virus infection and on days 3 and 5 p.i.
e
For AM depletion, mice received L-Cl2MBP i.n. on days −4 and −2 relative to the time of i.n. virus infection.