TABLE 2.
Summary of RNA protein binding data
| Probe | Sequenceb | % Binding | SD | Predicted effect on second structure |
|---|---|---|---|---|
| Wild type | 5′ AGUAAAUGAAUGAAGUUGAUC | 100 | NAa | NAa |
| AUGGCCAAUUGGAAGAAUCAC | ||||
| MT1A | 5′ AGUAAAaGAAUGAAGUUGAUC | 68.5 | 11.3 | None |
| AUGGCCAAUUGGAAGAAUCAC | ||||
| MT2A | 5′ AGUAAAacAAUGAAGUUGAUC | 64 | 21.7 | None |
| AUGGCCAAUUGGAAGAAUCAC | ||||
| MT3C | 5′ AGUAAAacuAUGAAGUUGAUC | 6.3 | 6.1 | Disrupts |
| AUGGCCAAUUGGAAGAAUCAC | ||||
| MT5A | 5′ AGUAAAacuuaGAAGUUGAUC | 25.2 | 5.5 | Disrupts |
| AUGGCCAAUUGGAAGAAUCAC | ||||
| MD10 | 5′ AGUAAAUGAAUGAAGUUGAUC | 44.1 | 2.5 | Disrupts |
| AUGGCCAAUUG | ||||
| M19C | 5′ AGUAAAacuAUGAAGUUGAUC | 74.1 | 8.6 | Restores |
| AUcGCCAAUUGGAAGAAUCAC | ||||
| 19C | 5′ AGUAAAUGAAUGAAGUUGAUC | 73.5 | 25.4 | None |
| AUcGCCAAUUGGAAGAAUCAC | ||||
| ATW5′ | 5′ AGUAAAUGAAUGAAGUacuaC | 7.2 | 8.7 | Disrupts |
| AUGGCCAAUUGGAAGAAUCAC | ||||
| ATW3′ | 5′ AGUAAAUGAAUGAAGUUGAUC | 400 | 84.4 | Disrupts |
| AUGGCCAAUUGGAAGAuaguC | ||||
| ATW | 5′ AGUAAAUGAAUGAAGUacuaC | 185.8 | 34.7 | Restores |
| AUGGCCAAUUGGAAGAuaguC |
a
NA, not applicable.
b
Mutations are indicated by lowercase type.