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Confocal microscope images of M. truncatula colonized by gfp-labeled M. extorquens. (A to C) Surfaces of the lower part of leaves. In panel C, bacteria are visible at the base of trichomes. (D to I) Interior of the leaves. (D) Epidermal cell layer. (E and F) Epidermal cell layer with stomata. (G and H) Mesophyll. Chloroplasts are visible due to their red autofluorescence. (I) Mesophyll. Cell walls and nuclei are stained red by propidium iodide. Bars = 20 μm.
