TABLE 1.

Oligonucleotide probes and primers used for in situ hybridization analyses and PCR reactions

Probe or primer	Sequence (5′ → 3′)	Target	% Formamidea	NaCl concn (mM) in washing buffer	Tdb	Reference
Probes
EUB 338	GCTGCCTCCCGTAGGAGT	Eubacteria	35	80	46	1
ARCH915	GTGCTCCCCCGCCAATTCCT	Archaea	20	200	46	38
SRB 385R	CGGCGTCGCTGCGTCAGG	SRB (δ-Proteobacteria)	35	88	46	34
660R	GAATTCCACTTTCCCCTCTG	Desulfobulbus	60	15	46	13
129F	CAGGCTTGAAGGCAGATT	Desulfobacter	20	15	46	13
687R	TACGGATTTCACTCCT	Desulfovibrio	10	15	46	13
MB1174	TACCGTCGTCCACTCCTTCCTC	Methanobacteriaceae	40		62	35
MSMX860	GGCTCGCCTCACGGCTTCCCT	Methanosarcinaceae	40		60	35
MG1200	CGGATAATTCGGGGCATGCTG	Methanomicrobiaceae	40		53	35
Primers
21f	TTCCGGTTGATCCYGCCGGA	Archaea				5
958r	YCCGGCGTTGAMTCCAATT	Archaea				5
27f	AGAGTTTGATCCTGGCTCAG	Eubacteria				18
1492r	TACCTTGTTACGACTT	Universal				18
907r	CCGTCAATTC(AC)TTT(AG)AGTTT	Universal				9

^aFormamide concentration in hybridization buffer.

^bHybridization temperature (°C).