TABLE 1.
Probe or primer | Sequence (5′ → 3′) | Target | % Formamidea | NaCl concn (mM) in washing buffer | Tdb | Reference |
---|---|---|---|---|---|---|
Probes | ||||||
EUB 338 | GCTGCCTCCCGTAGGAGT | Eubacteria | 35 | 80 | 46 | 1 |
ARCH915 | GTGCTCCCCCGCCAATTCCT | Archaea | 20 | 200 | 46 | 38 |
SRB 385R | CGGCGTCGCTGCGTCAGG | SRB (δ-Proteobacteria) | 35 | 88 | 46 | 34 |
660R | GAATTCCACTTTCCCCTCTG | Desulfobulbus | 60 | 15 | 46 | 13 |
129F | CAGGCTTGAAGGCAGATT | Desulfobacter | 20 | 15 | 46 | 13 |
687R | TACGGATTTCACTCCT | Desulfovibrio | 10 | 15 | 46 | 13 |
MB1174 | TACCGTCGTCCACTCCTTCCTC | Methanobacteriaceae | 40 | 62 | 35 | |
MSMX860 | GGCTCGCCTCACGGCTTCCCT | Methanosarcinaceae | 40 | 60 | 35 | |
MG1200 | CGGATAATTCGGGGCATGCTG | Methanomicrobiaceae | 40 | 53 | 35 | |
Primers | ||||||
21f | TTCCGGTTGATCCYGCCGGA | Archaea | 5 | |||
958r | YCCGGCGTTGAMTCCAATT | Archaea | 5 | |||
27f | AGAGTTTGATCCTGGCTCAG | Eubacteria | 18 | |||
1492r | TACCTTGTTACGACTT | Universal | 18 | |||
907r | CCGTCAATTC(AC)TTT(AG)AGTTT | Universal | 9 |
Formamide concentration in hybridization buffer.
Hybridization temperature (°C).