TABLE 2.
Phenotypic dataa and phylogenetic affiliations (based on 16S rRNA gene analysisb) of the cultured bacteria
| Isolatec/Anopheles sp. | GenBank accession no./CCUG no. | Growth temp interval (°C) (growth temp optimum [°C]/GT [min])d | Phylogenetic affiliation | Closest relative according to BLASTn (% identity) | Closest relative(s) according to phylogenetic analysis |
|---|---|---|---|---|---|
| B2.1B/arabiensis | AY837746/49717 | 10-40, (35/32) | Bacillaceae | Bacillus simplex AJ628747 (99) | Bacillus sp. strains AJ315059 and AJ315062 |
| E2.5/arabiensis | AY837747/49718 | 10-40, (35/20) | Vibrionaceae | Vibrio metschnikovii X74712 (98) | V. metschnikovii X74711 and X74712 |
| H2.1/arabiensis | AY837748/49520 | 15-45, (30/100) | γ-Proteobacteria | Serratia odorifera AJ233432 (91) | See Fig. 2A |
| H2.3/arabiensis | AY837749/49711 | 20-40, (40/104) | Nocardiaceae | Nocardia corynebacterioidese AF430066 (99) | See Fig. 3 |
| H2.5/arabiensis | AY837750/49712 | 10-40, (35/92) | Bacillales | Bacillus silvestris AJ006086 (99) | B. silvestris AJ006086 |
| H2.14/arabiensis | AY837751/49713 | 10-40, (35/28) | Enterobacteriaceae | Escherichia senegalensisAY217654f (98) | See Fig. 2B |
| H2.16B/arabiensis | AY837752/49715 | 20-40, (35/44) | Intrasporangiaceae | Janibacter limosus Y08539 (98) | Janibacter sp. strain AF170746 |
| H2.26/gambiae sensu stricto | AY837753/49716 | 10-35, (30/32) | Pseudomonadaceae | Pseudomonas putida AF447394 (99) | P. monteilii AF181576, P. mosselii AF072688 |
a
Additional data are available at http://www.ccug.se.
b
Sequence analyses are based on 1.4 to 1.5 kb and were performed in November 2004.
c
The mosquito label consists of two parts: first, the sampling occasion (A2 to H2), and second, a number in order of dissection. Isolates retrieved from a mosquito have the same label as the mosquito.
d
10°C was the lowest temperature examined. GT, generation time.
e
Synonyms Rhodococcus corynebacterioides and Nocardia corynebacteroides.
f
Best hit with sequence having a species name.