TABLE 2.

Relative abundances of phospholipids for B. subtilis estimated by MALDI-MS and THM-GC

Technique or combination	Relative abundance (%) of phospholipids or probable fatty acid combinationsa
	C30:0	C31:0	C32:0	Othersb	Total
On-probe MALDIc	53.7	14.2	32.0		100
THM-GCd	32.2	23.4	32.3	12.1	100
Combinatione	aC15:0, aC15:0; aC15:0, iC15:0	aC15:0, C16:0; iC15:0, C16:0	C16:0, C16:0; aC15:0, iC17:0

^aAbundance is shown based on the number of carbons and double bonds or rings in constituting acyl groups.

^bExamples of others are C28:0, C29:0, C33:0, etc.

^cAbundances were estimated from the peak intensities observed by MALDI-MS using on-probe sample pretreatment.

^dAbundances were calculated based on the fatty acid compositions for the same B. subtilis colony obtained by THM-GC.

^eCombinations were estimated based on the fatty acid distribution for the same B. subtilis colony sample by THM-GC.