TABLE 1.
Sample no. | Sampling
|
Initial no. of contaminated organisms (CFU/g)
|
Detection methoda
|
||||
---|---|---|---|---|---|---|---|
Day | Time | Salmonella | Bacteria | Culture | LAMP | PCR | |
1 | 1 | a.m. | 1.2 × 102 | 3.5 × 105 | + | + | + |
2 | p.m. | 1.3 × 102 | 9.2 × 104 | + | + | + | |
3 | 2 | a.m. | 1.0 × 101 | 1.8 × 106 | + | + | + |
4 | p.m. | <1 | 4.6 × 104 | + | + | + | |
5 | 3 | a.m. | 3.0 × 101 | 6.4 × 106 | + | + | + |
6 | p.m. | 3.0 × 101 | 7.9 × 104 | + | + | + | |
7 | 4 | a.m. | 4.0 × 101 | 2.0 × 106 | + | + | + |
8 | p.m. | <1 | 3.7 × 104 | + | + | + | |
9 | 5 | a.m. | 7.0 × 101 | 1.5 × 106 | + | + | + |
10 | p.m. | 3.0 × 101 | 9.1 × 104 | + | + | + | |
11 | 6 | a.m. | 1 | 1.4 × 106 | + | + | + |
12 | p.m. | 2 | 6.5 × 104 | + | + | + | |
13 | 7 | a.m. | <1 | 6.1 × 105 | + | + | + |
14 | p.m. | 3.0 × 101 | 1.1 × 105 | + | + | + | |
15 | 8 | a.m. | 7.0 × 101 | 4.5 × 106 | + | + | + |
16 | p.m. | 2.4 × 102 | 1.7 × 105 | + | + | + | |
17 | 9 | a.m. | 1 | 2.6 × 106 | + | + | + |
18 | p.m. | 3 | 1.7 × 105 | + | + | + | |
19 | 10 | a.m. | 2 | 4.3 × 106 | + | + | + |
20 | p.m. | 2.0 × 101 | 6.8 × 105 | + | + | + | |
21 | 11 | a.m. | <1 | 8.4 × 105 | + | + | + |
22 | p.m. | 8.0 × 101 | 2.2 × 105 | + | + | + | |
23 | 12 | a.m. | 2.1 × 102 | 1.6 × 107 | + | + | + |
24 | p.m. | 4 | 6.7 × 104 | + | + | + |
Culture consisted of enrichment culture in BPW with a 25-g sample, followed by culture with TT and RV, plated onto xylose lysine deoxycholate. For LAMP and PCR, enrichment culture in BPW with a 25-g sample was used for the assays.