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. 2026 Jan 23;16:1724684. doi: 10.3389/fimmu.2025.1724684

Figure 4.

Diagram illustrating bone marrow isolation and differentiation of cells into osteoclasts with timelines noted in hours post-infection (hpi). Bar graphs (B-D) show data on osteoclast (OC) number, diameter, and nuclei count at 72 and 120 hpi, comparing non-infected, wild type, and ΔdotA conditions. Statistical significance is indicated with p-values.

C. burnetii infection of myeloid progenitor cells inhibits osteoclast differentiation. (A) Osteoclast progenitors were infected with C. burnetii WT or ΔdotA before being stimulated with RANKL (6 hpi). Osteoclast differentiation was analyzed 72 hpi and 120 hpi (matching time point with the previous experiment presented in Figure 3A) by staining for TRAP. Representative microscopy images are presented in Supplementary Figure 3. (B) Osteoclasts number, (C) diameter and (D) nuclei number were quantified (n=2). Error bars represent the standard deviation. Data are shown as mean ± SD. Circle represent individual microscopical fields of view.